Composite
Part:BBa_K2637021:Design
Designed by: Haoqian Zhao Group: iGEM18_Tianjin (2018-10-05)
AD-KaiC, Gal4 activation domain fused with KaiC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 670
Illegal BglII site found at 1177 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Gal4 activation domain fused with KaiC gene. KaiC has been optimized in yeast.
Source
Gal4 activation domain is from yeast genome and KaiC is from Synechococcus elongatus PCC7942.