Composite

Part:BBa_K2637021:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)


AD-KaiC, Gal4 activation domain fused with KaiC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BglII site found at 1177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gal4 activation domain fused with KaiC gene. KaiC has been optimized in yeast.

Source

Gal4 activation domain is from yeast genome and KaiC is from Synechococcus elongatus PCC7942.

References